InforLorV4, Main, Exploration, bibRecord, 005273

Crystallization and characterization of the thallium form of the Oxytricha nova G-quadruplex

Identifieur interne : 005273 ( Main/Exploration ); précédent : 005272; suivant : 005274

Crystallization and characterization of the thallium form of the Oxytricha nova G-quadruplex

Auteurs : Michelle L. Gill [États-Unis] ; Scott A. Strobel [États-Unis] ; J. Patrick Loria [États-Unis]

Source :

Nucleic Acids Research [ 0305-1048 ] ; 2006.

RBID : PMC:1636370

Abstract

The crystal structure of the Tl⁺ form of the G-quadruplex formed from the Oxytricha nova telomere sequence, d(G₄T₄G₄), has been solved to 1.55 Å. This G-quadruplex contains five Tl⁺ ions, three of which are interspersed between adjacent G-quartet planes and one in each of the two thymine loops. The structure displays a high degree of similarity to the K⁺ crystal structure [Haider et al. (2002), J. Mol. Biol., 320, 189–200], including the number and location of the monovalent cation binding sites. The highly isomorphic nature of the two structures, which contain such a large number of monovalent binding sites (relative to nucleic acid content), verifies the ability of Tl⁺ to mimic K⁺ in nucleic acids. Information from this report confirms and extends the assignment of ²⁰⁵Tl resonances from a previous report [Gill et al. (2005), J. Am. Chem. Soc., 127, 16 723–16 732] where ²⁰⁵Tl NMR was used to study monovalent cation binding to this G-quadruplex. The assignment of these resonances provides evidence for the occurrence of conformational dynamics in the thymine loop region that is in slow exchange on the ²⁰⁵Tl timescale.

Url:

http://www.ncbi.nlm.nih.gov/pmc/articles/PMC1636370

DOI: 10.1093/nar/gkl616
PubMed: 16945956
PubMed Central: 1636370

Affiliations:

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<front><div type="abstract" xml:lang="en"><p>The crystal structure of the Tl<sup>+</sup>
 form of the G-quadruplex formed from the <italic>Oxytricha nova</italic>
 telomere sequence, d(G<sub>4</sub>
T<sub>4</sub>
G<sub>4</sub>
), has been solved to 1.55 Å. This G-quadruplex contains five Tl<sup>+</sup>
 ions, three of which are interspersed between adjacent G-quartet planes and one in each of the two thymine loops. The structure displays a high degree of similarity to the K<sup>+</sup>
 crystal structure [Haider <italic>et al.</italic>
 (2002), <italic>J. Mol. Biol.</italic>
, 320, 189–200], including the number and location of the monovalent cation binding sites. The highly isomorphic nature of the two structures, which contain such a large number of monovalent binding sites (relative to nucleic acid content), verifies the ability of Tl<sup>+</sup>
 to mimic K<sup>+</sup>
 in nucleic acids. Information from this report confirms and extends the assignment of <sup>205</sup>
Tl resonances from a previous report [Gill <italic>et al.</italic>
 (2005), <italic>J. Am. Chem. Soc.</italic>
, 127, 16 723–16 732] where <sup>205</sup>
Tl NMR was used to study monovalent cation binding to this G-quadruplex. The assignment of these resonances provides evidence for the occurrence of conformational dynamics in the thymine loop region that is in slow exchange on the <sup>205</sup>
Tl timescale.</p>
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Crystallization and characterization of the thallium form of the Oxytricha nova G-quadruplex

Crystallization and characterization of the thallium form of the Oxytricha nova G-quadruplex

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